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Elisa Kits
Leading Manufacturer of jmitra hcv microlisa 3rd generation elisa kit, jmitra hcv tri-dot rapid test, calbiotech dengue elisa test kit, jmitra hbv hepa elisa hepalisa test kit, hbsag rapid card test is it medsource ozone and maglumi ft4 kit from Jaipur.
Jmitra HCV Microlisa 3rd Generation Elisa Kit
Product Price: Rs 2,100 / PieceGet Best Price
Minimum Order Quantity: 1 Piece
Product Brochure
Product Details:
| ICMR Approved | Yes |
| Brand | J.mitra |
| Usage/Application | Laboratory |
| Sample Type | Blood |
| Product Type | Elisa Kit |
| Packaging Type | 96 Test |
| Pack Type | Box |
| Country of Origin | Made in India |
Salient Features
- 3rd Generation Assay
- Unique combination of HCV antigens for E1, E2, Core, NS3, NS4 & NS5
- Break-away microwell strips
- Based on Indirect Elisa principle
- Colour coded reagents to monitor procedural steps.
- Short assay procedure: 90mins
- Longer shelf life: 24 months at 2-8°C.
- Sensitivity 100% & Specificity 97.4% by WHO.
Principle
The 3rd generation HCV Microlisa is based on a highly sensitive technique, Enzyme linked Immunosorbent Assay which detects antibodies against HCV in human serum and plasma. The HCV proteins are present in serum at levels well below the limits of detection. Thus immunodiagnosis of HCV infection is based on detection of host generated antibodies (anti-HCVs) to viral proteins. The 3rd generation HCV Microlisa utilizes a combination of antigen with the sequence of both HCV structural and non-structural antigens i.e. CORE, E1, E2, NS3, NS4 and NS5. It has an obvious advantage over the available 2nd Generation and 1st Generation Elisa with improved sensitivity and specificity. The combination of antigens for the structural and non-structural HCV proteins is coated onto the microwells. Diluted sample and controls are then incubated. Antibodies to HCV, if present, bind to the immobilized HCV antigens on the microwell during this incubation period.
The microwells are then thoroughly washed with the diluted wash buffer to remove excess of unbound anti-HCV or other human IgGs which may interfere with the test. An enzyme conjugate, anti-human IgG conjugated with HRPO is added. The excess of enzyme conjugate is again removed with diluted wash buffer. At this stage the microwells hold only the bound antigen-anti HCV-enzyme conjugate complex. In the next step, the freshly prepared substrate solution is incubated with the complex in the microwells. The enzyme substrate reaction leads to development of a blue colour which is indicative of the Ag-Ab reaction which has occurred in the microwell. In the final step the stop solution is added and the optical density of the developed colour is read at 450nm spectrophotometrically.
Ordering Information| Product Name | Pack Size | Catalog No. |
|---|---|---|
| 3rd generation HCV Microlisa | 96 Tests | HC023096 |
- WHO EVALUATION: The kit has been evaluated by WHO Collaborating Centers by using various panels from Latin America, Asia, European, African origin, Various Sero conversion panels from BBI, Worldwide performance panel. The Evaluation indicates the following sensitivity and specificity: Sensitivity 100% & Specificity 97.4%
- CENTER FOR LIVER DISEASES –HYDERABAD- The overall result showed 100% Sensitivity & 100% Specificity.
- The kit has also been evaluated from National Institute of Virology-Pune & National Institute of Cholera & Enteric diseases and the overall Sensitivity & Specificity was found to be 100 % .
- Approved by Drug Controller General of India( DCGI) for the usage in Blood Banks.
Additional Information:
- Delivery Time: 1 Day
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Jmitra HCV Tri-dot Rapid Test
Product Price: Rs 55 / PieceGet Best Price
Minimum Order Quantity: 100 Piece
Product Brochure
Product Details:
| Type | Rapid test kits |
| ICMR Approved | Yes |
| Brand | Jmitra |
| Sample Type | Blood |
| Result Time | 3 Minutes |
| Shelf Life | 24 Months |
| Packaging Type | Box |
| Country of Origin | Made in India |
The 4th Generation HCV TRI-DOT is a rapid, visual, sensitive and qualitative in vitro diagnostic test for the detection of (IgM,IgG & IgA) antibodies to Hepatitis C virus in human serum or plasma.
It has been developed and designed with increased sensitivity for core and NS3 antibodies using a unique combination of modified HCV antigen.
- Based on “Flow Through Technology”, which is similar to Elisa technology because of involvement of washing steps at various levels to enhance the specificity.
- Use of highly purified HCV antigens for Core, NS3, NS4, NS5 immobilized on the device.
- Detection of all the subtypes of HCV.
- Results within 3 minutes.
- In built quality control dot which validates the test.
- 100% Sensitivity & 98.9% Specificity as per WHO evaluation.
- Shelf life : 24 months at 2-8°C
- HCV antigens are immobilized on a porous immunofiltration membrane. Sample and the reagents pass through the membrane and are absorbed into the underlying absorbent pad.
- As the patient’s sample passes through the membrane, HCV antibodies if present in serum/plasma, bind to the immobilized antigens. In the subsequent washing step, unbound serum/ plasma proteins are removed.
- In the next step, the protein-A conjugate is added which binds to the Fc portion of the HCV antibodies to give distinct pinkish purple dot against a white background at the test region (“T1” &/or “T2”). At the control region (“C”) a “Built-in Quality Control Dot” has been devised to confirm the proper functioning of the device, reagent and correct procedural application.
Additional Information:
- Delivery Time: 1 Day
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Calbiotech Dengue Elisa Test Kit
Product Price: Rs 9,800 / PieceGet Best Price
Product Brochure
Product Details:
| ICMR Approved | Yes |
| Brand | Calbiotech USA |
| Sterile/ Non Sterile | Sterile |
| Number of Reactions(Preps)/Kit | 25 |
| Result Time (Rapid Kits) | 15MIN |
| Packaging Type | Box |
| Usage/Application | Hospital |
| Disposable/ Reusable | Disposable |
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Calbiotech Dengue Elisa Test Kit
Product Price: Rs 9,800 / BoxGet Best Price
Minimum Order Quantity: 1 Box
Product Brochure
Product Details:
| ICMR Approved | Yes |
| Brand | Calbiotech |
| Usage/Application | Laboratory |
| Packaging Type | 96 Test |
| Sample Type | Blood |
| Product Type | Elisa Kits |
| Test Type | Elisa |
| Packing Type | Box |
| Country of Origin | Made in India |
| Catalog number |
DE050G |
| Product type | ELISA |
| Quantity |
96 Tests (12x8 breakable strip wells) |
| Sample volume | 10 µl/well |
| Species | Human |
| Storage and Stability | Product should be stored at 2-8 °C. |
| Precautions | For research use only. Not for use in diagnostic procedures. |
| References | 1. Pinheiro FP, Corber SJ: Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Stat Q 50(3/4):161-169, 1997. 2. Gubler DJ, Trent DW: Emergence of epidemic dengue/dengue hemorrhagic fever as a public health problem in the Americas. Infect Agents Dis 2:383-393, 1993. 3. Wu SJ; Hanson B; Paxton H; Nisalak A; Vaughn DW; Rossi C; Henchal EA; Porter KR; Watts DM; Hayes CG. Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus. Clin Diagn Lab Immunol1997; 4(4):452-7. 4. Lam SK; Devine PL. Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infection. Clin Diagn Virol 1998;10(1):75-8. 5. Rossi CA; Drabick JJ; Gambel JM; Sun W; Lewis TE; Henchal EA. Laboratory diagnosis of acute dengue fever during the United Nations Mission in Haiti, 1995-1996. Am J Trop Med Hyg 1998;59(2):275-8 |
Additional Information:
- Delivery Time: 1 Day
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Jmitra HBV Hepa Elisa Hepalisa Test Kit
Product Price: Rs 1,200 / KitGet Best Price
Minimum Order Quantity: 1 Kit
Product Brochure
Product Details:
| ICMR Approved | Yes |
| Brand | Jmitra |
| Usage/Application | Laboratory |
| Disposable/ Reusable | Disposable |
| Sample Type | Blood |
| Product Type | Elisa Hepalisa Test Kit |
| Packaging Type | Box |
| Country of Origin | Made in India |
Salient Features
- Detects all the known 11-subtypes of HBV
- Antigen Sensitivity-0.1ng/ml.
- Two step Immunoassay.
- Short protocol: Based on single washing step.
- Assay Procedure: 90 mins.
- Shelf life-24 months at 2-8°C.
- Convenient pack size :96 tests
- Break-away wells for minimal wastage
- Easy interpretation of result
- Sensitivity & Specificity 100% by WHO.
Hepalisa is a solid phase enzyme linked immunosorbent assay based on the Direct Sandwich Elisa principle. The microwells are coated with Monoclonal antibodies with high reactivity for HBsAg. The samples are added in the wells followed by addition of enzyme conjugate (polyclonal antibodies linked to Horseradish Peroxidase (HRPO). A sandwich complex is formed in the well wherein HBsAg (from serum sample) is “trapped” or “sandwiched” between the antibody and antibody HRPO conjugate. Unbound conjugate is then washed off with wash buffer.
The amount of bound peroxidase is proportional to the concentration of HBsAg present in the sample. Upon addition of the substrate buffer and chromogen, a blue colour develops. The intensity of developed blue colour is proportional to the concentration of HBsAg in sample. To limit the enzyme-substrate reaction, stop solution is added and a yellow colour develops which is finally read at 450 nm spectrophotometrically.
Ordering Information| Product Name | Pack Size | |
|---|---|---|
| Hepalisa | 96 Tests |
- WHO EVALUATION: The kit has been evaluated by WHO Collaborating Centers by using a panel of 276 sera consisting of samples from Latin America, Asia, European, African origin , Various sero-conversion panels from BBI , worldwide performance panel and one HBsAg low titer performance panel. The Evaluation indicates the following sensitivity and specificity: Sensitivity- 100 % & Specificity-100 %
- BANGALORE MEDICAL COLLEGE: The overall Sensitivity & Specificity was found to be 100%
- The kit has also been evaluated from National Institute of Virology-Pune & National Institute of Cholera & Enteric diseases and the overall Sensitivity & Specificity was found to be 100 % .
- Approved by Drug Controller General of India( DCGI) for the usage in Blood Banks.
Additional Information:
- Delivery Time: 1 Day
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Jmitra HBV Hepa Elisa Hepalisa Test Kit
Product Price: Rs 1,200 / PieceGet Best Price
Product Brochure
Product Details:
| Type | Direct ELISA |
| ICMR Approved | Yes |
| Usage/Application | Clinic |
| Number of Reactions(Preps)/Kit | 20 |
| Sterile/ Non Sterile | Sterile |
| Disposable/ Reusable | Disposable |
| Result Time (Rapid Kits) | 20 sec |
| Sample Type | Blood |
| Country of Origin | Made in India |
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HBsAg Rapid Card Test is it Medsource Ozone
Product Price: Rs 500 / BoxGet Best Price
Product Details:
| ICMR Approved | Yes |
| Sample Type | Blood |
| Country of Origin | Made in India |
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Maglumi FT4 kit
Product Price: Rs 100 / PieceGet Best Price
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Maglumi LH Kit
Product Price: Rs 100 / KitGet Best Price
Maglumi LH kit
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Maglumi T4 Kit
Product Price: Rs 100 / PieceGet Best Price
Maglumi T4 kit
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